: 0522-2497903        : cif@niperraebareli.edu.in

UV-Vis Spectrophotometer [Cary 100 Win UV-Vis Spectrophotometer, Agilent Technologies] with Thermal Control

UV-vis spectrophotometer measures changes in absorbance of a molecule as a function of several parameters such as wavelength, time, concentration etc in addition to determining the intrinsic optical properties of the sample under investigation. It has a number of applications in biosciences, chemical and material sciences as well as in the pharmaceutical sciences for quality control. The Cary 100 UV-visible instrument housed in our central facility is equipped with a 12 cell multi-cell holder and a peltier thermal controller to permit temperature dependant analysis of up to 12 samples concurrently. Apart from the regular analysis of intrinsic properties of the samples, this machine can be used to understand a number of drug-biomolecule interaction processes by observing the changes in the absorbance with respect to the amount of drug being added. It also helps to analyze the changes in the microenvironment of the biomolecule upon drug binding. It pharmaceutical industry, UV-Visible spectrophotometers are integral part of the quality control and used routinely in the analysis of drug samples.

The UV-Visible spectrophotometer present in our facility allows following analysis-

  1. Scan: Scanning of samples at a specified wavelength range.
  2. Simple Read: Scanning of samples at a particular wavelength.
  3. Advanced Read: scanning of multiple samples at single or multiple wavelengths in a single scan.
  4. Concentration: Quantitative measurement of sample
  5. Kinetics: Gives absorption versus time data to calculate rate of reaction and half-life
  6. Thermal Melting: Thermal denaturing of nucleic acids and protein as function of temperature.